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Microscopy Based Methods for the Assessment of Epithelial Cell Migration During In Vitro Wound Healing
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JoVE Journal Medicina
Microscopy Based Methods for the Assessment of Epithelial Cell Migration During In Vitro Wound Healing

Microscopy Based Methods for the Assessment of Epithelial Cell Migration During In Vitro Wound Healing

DOI:
10.3791/56799-v

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08:34 min

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January 02, 2018

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, , ,
1Laboratorio de Oncología Molecular y TGF-β,IMIB-Arrixaca, 2Departamento de Enfermería, Facultad Enfermería,Universidad de Murcia

Capítulos

  • 00:05Título
  • 01:07Artificial Wound Scratch Assay
  • 03:19Artificial Wound Image Analysis and Migration Quantification
  • 04:48Artificial Migration Front Assay
  • 07:07Results: Representative Artificial Wound Scratch and Migration Front Assay Imaging
  • 08:00Conclusion

Summary

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Tadução automática

This manuscript describes how incision-like lesions made on cultured epithelial cell monolayers conveniently model wound healing in vitro, allowing for imaging by confocal or laser scanning microscopy, and which can provide high-quality quantitative and qualitative data for studying both cell behavior and the mechanisms involved in migration.

Tags

Epithelial Cell MigrationIn Vitro Wound HealingArtificial WoundingCell MigrationWound HealingMolecular MarkersArtificial Wound Scratch AssayEpithelial CellsConfluencySerum-free MediumMicro-pipetteWound GapPhase Contrast MicroscopeCCD CameraWound Treatments

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