Journal
/
Química
/
邻苯二甲酸酯结合 DNA 核酸适体的选择、表征及其在电化学 Aptasensor 中的应用
JoVE Journal
Química
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Química
Phthalic Acid Ester-Binding DNA Aptamer Selection, Characterization, and Application to an Electrochemical Aptasensor
Please note that all translations are automatically generated. Click here for the English version.

邻苯二甲酸酯结合 DNA 核酸适体的选择、表征及其在电化学 Aptasensor 中的应用

DOI:
10.3791/56814-v

•

09:33 min

•

March 21, 2018

•

, , , , ,
1Department of Chemistry,Capital Normal University, 2College of Life Sciences,Capital Normal University

Capítulos

  • 00:04Título
  • 00:50Small-scale λ Exonuclease Reaction
  • 02:40Relative Affinity and Specificity Test by Competitive Assays
  • 04:17Fabrication and Electrochemical Measurements of Diethylhexyl Phthalate (DEHP) Electrochemical Biosensors
  • 07:52Results: Optimization of λ Exonuclease Reaction and Electrochemical Aptasensor Evaluation
  • 08:46Conclusion

Summary

Tadução automática

Tadução automática

本文提出了一种体外的协议, 用于对组特定邻苯二甲酸酯结合 DNA 适配子的筛选和表征。在电化学 aptasensor 中也包括了所选核酸适体的应用。

Tags

Phthalic Acid EsterDNA AptamerElectrochemical AptasensorHydrophobic MoleculesGroup-specific AptamersLambda ExonucleaseNative PAGE GelMagnetic BeadsPAE Binding Buffer

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

check_url/pt/56814?article_type=v

折叠的DNA折纸一个生物反应机器人与表征
check_url/pt/56814?article_type=v

核苷三磷酸 - 从合成到生化特征
check_url/pt/56814?article_type=v

一个反分析方法化工运输的油漆表征
check_url/pt/56814?article_type=v

氯离子的动态电化学测试
check_url/pt/56814?article_type=v

对于未螯合钆基于核酸适配体传感器（III）
check_url/pt/56814?article_type=v

电化学阻抗谱作为电化学速率常数估计的一种工具
check_url/pt/56814?article_type=v

一种用于快速和低压彩色调制的电化学胆固醇液晶器件
check_url/pt/56814?article_type=v

薄膜铂金宏观和微电极的电化学粗加工
check_url/pt/56814?article_type=v

用于在等温操作下收集低档热量的不对称热电化学电池
check_url/pt/56814?article_type=v

使用混合原子力显微镜扫描电化学显微镜（AFM-SECM）探测纳米材料的表面电化学活性

Read Article