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Phthalater syre Ester-bindende DNA Aptamer udvalg, karakterisering og ansøgning til en elektrokemisk Aptasensor
JoVE Journal
Chemistry
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JoVE Journal Chemistry
Phthalic Acid Ester-Binding DNA Aptamer Selection, Characterization, and Application to an Electrochemical Aptasensor
DOI:

09:33 min

March 21, 2018

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Chapters

  • 00:04Title
  • 00:50Small-scale λ Exonuclease Reaction
  • 02:40Relative Affinity and Specificity Test by Competitive Assays
  • 04:17Fabrication and Electrochemical Measurements of Diethylhexyl Phthalate (DEHP) Electrochemical Biosensors
  • 07:52Results: Optimization of λ Exonuclease Reaction and Electrochemical Aptasensor Evaluation
  • 08:46Conclusion

Summary

Automatic Translation

En protokol til in vitro- udvælgelse og karakterisering af gruppespecifikke phthalater syre ester-bindende DNA aptamers præsenteres. Anvendelsen af den valgte aptamer i en elektrokemisk aptasensor er også inkluderet.

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