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核糖体剖面对芽殖酵母中翻译的全基因组定量

JoVE Journal
Bioquímica

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JoVE Journal Bioquímica

Genome-wide Quantification of Translation in Budding Yeast by Ribosome Profiling

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核糖体剖面对芽殖酵母中翻译的全基因组定量

DOI:

10.3791/56820-v

•

12:57 min

•

December 21, 2017

•

Carine Beaupere, Rosalyn B. Chen, William Pelosi, Vyacheslav M. Labunskyy

¹Department of Dermatology,Boston University School of Medicine

Capítulos

00:05Título
00:52Extract Preparation
02:43Footprint Extraction
05:15Dephosphorylation
07:07Reverse Transcription
10:17Results: Ribo-seq Enables Genome-wide Quantification of Translation of Specific mRNAs Under Different Cellular Conditions
11:59Conclusion

Summary

Tadução automática

翻译调控在蛋白质丰度控制中起着重要的作用。在这里, 我们描述一个高通量的方法, 定量分析翻译在萌芽酵母酿酒酵母。

Tags

Ribosome Profiling Genome-wide Translation Budding Yeast Saccharomyces Cerevisiae Protein Translation Translational Regulation Cell Lysate Poly(A) MRNA Sucrose Gradient RNase I Cycloheximide

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