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基于3D 荧光显微镜的病毒转录工厂功能成像
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Biologia
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JoVE Journal Biologia
Functional Imaging of Viral Transcription Factories Using 3D Fluorescence Microscopy
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基于3D 荧光显微镜的病毒转录工厂功能成像

DOI:
10.3791/56832-v

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09:03 min

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January 18, 2018

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, ,
1Department of Dermatology, University of California Davis School of Medicine,University of California, Davis, 2Center for Biophotonics, Department of Biochemistry and Molecular Medicine,University of California, Davis

Capítulos

  • 00:05Título
  • 00:39Fixation and Permeabilization
  • 03:08IFA and RNA-FISH
  • 06:59Results: Viral Transcription Clearly Visible
  • 08:39Conclusion

Summary

Tadução automática

Tadução automática

病毒转录工厂是分散的结构, 富含细胞 RNA 聚合酶 II, 以增加病毒基因转录在重新激活。本文介绍了一种通过免疫荧光染色和原位RNA 杂交技术在3D 核空间中主动转录病毒染色质位置的方法。

Tags

Viral Transcription Factories3D Fluorescence MicroscopyNascent RNA-FISHAmino Acid StainingViral Latent ProteinsGenomic Architectural AlterationsInducible Viral Mini-chromosome ModelCell FixationCell PermeabilizationPrimary Antibody Labeling

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