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代谢图谱: 定量酶化学和组织化学法测定脱氢酶在细胞和器官中的活性
JoVE Journal
Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Metabolic Mapping: Quantitative Enzyme Cytochemistry and Histochemistry to Determine the Activity of Dehydrogenases in Cells and Tissues
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代谢图谱: 定量酶化学和组织化学法测定脱氢酶在细胞和器官中的活性

DOI:
10.3791/56843-v

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08:36 min

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May 26, 2018

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, , ,
1Cancer Center Amsterdam, Department of Medical Biology, Academic Medical Center,University of Amsterdam, Amsterdam, The Netherlands

Capítulos

  • 00:00Título
  • 00:50Preparing Reaction Buffer
  • 03:50Applying Incubation Medium to Tissue Sections
  • 05:32Washing the Microscopy Slides
  • 06:33Enclose the Tissue Sections on Microscopy Slides
  • 07:13Representative Results
  • 08:14Conclusion

Summary

Tadução automática

Tadução automática

在这里, 我们提出了一个协议, 可用于显微镜下可视化和量化的活动脱氢酶在细胞和组织及其动力学, 功能和亚细胞定位。

Tags

Keywords: Metabolic MappingEnzyme CytochemistryHistochemistryDehydrogenase ActivityCellsPolyvinyl AlcoholPVAPhosphate BufferNitroblue Tetrazolium ChlorideNitroBTEthanolDimethylformamideReaction BufferCytospinsMetabolismCancer CellsTherapeutic Target

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