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An In Vivo Murine Sciatic Nerve Model of Perineural Invasion
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Pesquisa do Câncer
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JoVE Journal Pesquisa do Câncer
An In Vivo Murine Sciatic Nerve Model of Perineural Invasion

An In Vivo Murine Sciatic Nerve Model of Perineural Invasion

DOI:
10.3791/56857-v

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09:55 min

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April 23, 2018

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, , , , , ,
1Department of Surgery,Memorial Sloan Kettering Cancer Center, 2Molecular Cytology Core Facility,Memorial Sloan Kettering Cancer Center, 3Department of Radiation Oncology,Mount Sinai Hospital

Capítulos

  • 00:05Título
  • 01:25Preparation of Cultured Cancer Cells
  • 02:35Preparation of Mouse and Surgery
  • 06:06Sciatic Nerve Extraction
  • 07:24Nerve Processing and Quantification
  • 08:26Results: H&E Longitudinal Sections of Sciatic Nerves Injected with Panc02H7 Cancer Cells in Wild Type and NCAM KO Mice
  • 09:10Conclusion

Summary

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We describe an in vivo murine model of perineural invasion by injecting syngeneic pancreatic cancer cells into the sciatic nerve. The model allows for quantification of the extent of nerve invasion, and supports investigation of the cellular and molecular mechanisms of perineural invasion.

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