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Simpel Generation af højtydende kultur af inducerede neuroner fra menneskelige voksen hud fibroblaster
JoVE Journal
Neurociência
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JoVE Journal Neurociência
Simple Generation of a High Yield Culture of Induced Neurons from Human Adult Skin Fibroblasts

Simpel Generation af højtydende kultur af inducerede neuroner fra menneskelige voksen hud fibroblaster

DOI:
10.3791/56904-v

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09:07 min

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February 05, 2018

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, , , ,
1Department of Experimental Medical Science, Wallenberg Neuroscience Center, Division of Neurobiology and Lund Stem Cell Center,Lund University, 2John van Geest Centre for Brain Repair & Department of Neurology, Department of Clinical Neurosciences and Cambridge Stem Cell Institute,University of Cambridge

Capítulos

  • 00:04Título
  • 01:02Preparation of Skin Fibroblasts for Reprogramming
  • 01:43Plating for Reprogramming (Day 1)
  • 03:22Viral Transduction (Day 0)
  • 04:12Maintenance of the Converting Cells
  • 04:58FACS
  • 07:33Results: Generation of Induced Neurons from Adult Human Dermal Fibroblasts
  • 08:15Conclusion

Summary

Tadução automática

Tadução automática

Direkte neuronal omprogrammering genererer neuroner, der opretholder en alder af det udgangspunktet i somatiske celler. Her, beskriver vi en enkelt vektor-baseret metode til at generere induceret neuroner fra dermal fibroblaster fremstillet af voksne menneskelige donorer.

Tags

Induced NeuronsHuman Adult Skin FibroblastsCell ReprogrammingNeurological DisordersDisease ModelingToxicologyDiagnosticsDrug ScreeningCell CultureFibroblast MediumGelatin CoatingCell SeedingCell CountingCell LysisTrypsinizationCell Suspension

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