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Zika Virus Infection of Cultured Human Fetal Brain Neural Stem Cells for Immunocytochemical Analysis
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Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Zika Virus Infection of Cultured Human Fetal Brain Neural Stem Cells for Immunocytochemical Analysis

Zika Virus Infection of Cultured Human Fetal Brain Neural Stem Cells for Immunocytochemical Analysis

DOI:
10.3791/56917-v

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10:27 min

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February 05, 2018

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, ,
1Department of Neuroscience and Cell Biology,University of Texas Medical Branch, 2Department of Neurology,Johns Hopkins University, 3Beijing Institute for Brain Disorders,Capital Medical University

Capítulos

  • 00:04Título
  • 00:57hNSC Passage
  • 04:50Plating Adherent hNSC for Validation
  • 06:02Zika Virus Infection
  • 07:37Results: Human Fetal Brain Neural Stem Cell Infection with Zika Virus
  • 09:03Conclusion

Summary

Tadução automática

Tadução automática

This article details the methods that are used to expand human fetal brain neural stem cells in culture, as well as how to differentiate them into various neuronal subtypes and astrocytes, with an emphasis on the use of neural stem cells to study Zika virus infection.

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Zika VirusHuman Fetal Brain Neural Stem CellsImmunocytochemical AnalysisIn VitroNeural Stem Cell DifferentiationInnate Immune ResponseTranslational ApplicationNeurological DeficitsCell CultureCell PassageTrypsinDNaseCentrifugation

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