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Methods for Staging Pupal Periods and Measurement of Wing Pigmentation of Drosophila guttifera
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Biologia
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JoVE Journal Biologia
Methods for Staging Pupal Periods and Measurement of Wing Pigmentation of Drosophila guttifera

Methods for Staging Pupal Periods and Measurement of Wing Pigmentation of Drosophila guttifera

DOI:
10.3791/56935-v

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08:03 min

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January 24, 2018

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, , ,
1Graduate School of Science,Kyoto University, 2Graduate School of Science,Osaka City University, 3The Hakubi Center for Advanced Research,Kyoto University, 4Graduate School of Environmental Science,Hokkaido University

Capítulos

  • 00:05Título
  • 00:46Puparium Removal
  • 02:13Image Preparation
  • 03:55Definition of Point A
  • 06:18Measurements
  • 07:19Results: Pigmentation Timing Identified
  • 07:37Conclusion

Summary

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Protocols for staging pupal periods and measurement of wing pigmentation of Drosophila guttifera are described. Staging and quantification of pigmentation provide a solid basis for studying developmental mechanisms of adult traits and enable interspecific comparison of trait development.

Tags

Keywords: Drosophila GuttiferaPupal PeriodWing PigmentationPuparium RemovalForcepsPhosphate-buffered SalineAnesthetizationWing Measurement

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