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Capítulos
- 00:05Título
- 01:18Metabolic Incorporation of Chemical Reporters
- 03:29Dual Fluorescence Labeling of Plant Cross-section Samples by SPAAC and CuAAC
- 06:08Mounting of Samples on Microscope Slides
- 07:49Results: Study of Lignification Dynamics via BLISS
- 09:40Conclusion
BLISS, a dual labeling protocol for studying lignification dynamics, was developed. Using synthetic monolignol reporters and a sequential combination of SPAAC and CuAAC bioorthogonal click reactions, this methodology paves the way to in-depth analysis of the factors that regulate the biogenesis of lignins in planta.
