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JoVE Journal Biochemistry
Combining X-Ray Crystallography with Small Angle X-Ray Scattering to Model Unstructured Regions of Nsa1 from S. Cerevisiae
 

Combining X-Ray Crystallography with Small Angle X-Ray Scattering to Model Unstructured Regions of Nsa1 from S. Cerevisiae

Article DOI: 10.3791/56953-v • 09:15 min • January 10th, 2018
January 10th, 2018 •
1, 1, 1
1Signal Transduction Laboratory, National Institutes of Environmental Health Sciences, Department of Health and Human Services, National Institutes of Health

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This method describes the cloning, expression, and purification of recombinant Nsa1 for structural determination by X-ray crystallography and small-angle X-ray scattering (SAXS), and is applicable for the hybrid structural analysis of other proteins containing both ordered and disordered domains.

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Keywords: X-ray Crystallography Small Angle X-ray Scattering (SAXS) Structural Analysis Protein Structure Recombinant Protein Unstructured Regions Nsa1 S. Cerevisiae Hybrid Structural Analysis Protein Function Protein Dynamics Protein Aggregation Purification Affinity Chromatography Gel Filtration SDS-PAGE TEV Protease Centrifugal Filtration
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