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Trans-inner Cell Mass Injection of Embryonic Stem Cells Leads to Higher Chimerism Rates
 

Trans-inner Cell Mass Injection of Embryonic Stem Cells Leads to Higher Chimerism Rates

Article DOI: 10.3791/56955-v • 05:53 min • May 29th, 2018
May 29th, 2018 •
1, 1, 1, 1
1Knock Out Mouse Core, NIEHS/NIH

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Here we present a protocol to increase chimera production without the use of new equipment. A simple orientation change of the embryo for injection can increase the number of embryos produced, and potentially reduce the timeline to germline transmission.

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Trans-inner Cell Mass Injection Embryonic Stem Cells Chimerism Rates Modified Procedure Increase Chimeric Production Efficient Chimera Production ES Cells Technique Additional Equipment Specialized Training Thomas Hagler Biologist Graduate Student Core Lab Harvest Embryos Blot Uterus Excess Blood 60 Millimeter Dish Low Magnification Dissecting Microscope Dissecting Forceps 25 Gauge Needle 10 Milliliter Syringe Blast Collection Medium Uterus Oviduct Parallel Plane Pressure Forceps Flush Medium Uterine Horn Blastocysts Expelled
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