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肿瘤组织中高通量芯片测序的染色质状态全基因组图谱集成平台
JoVE Journal
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An Integrated Platform for Genome-wide Mapping of Chromatin States Using High-throughput ChIP-sequencing in Tumor Tissues
DOI:

10:41 min

April 05, 2018

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Capítulos

  • 00:04Título
  • 00:31Tissue/Cell Line Processing and Cross-linking
  • 01:57Tissue Lysis, Sonication, and Antibody Preparation
  • 02:52Chromatin Immunoprecipitation
  • 03:40Washing and Reverse Cross-linking of Immunoprecipitated DNA-protein Complexes
  • 04:43Purification and Quantification of Precipitated DNA
  • 06:00Library Generation Using the NEBNext Ultra II DNA Library Preparation Kit
  • 07:53Results: Immunoprecipitation of Tumor Tissues and Cells Using High-throughput ChIP-sequencing
  • 09:23Conclusion

Summary

Tadução automática

在这里, 我们描述了一个优化的高通量芯片排序协议和计算分析管道, 以确定从冷冻肿瘤组织和细胞系的全基因组的染色质状态模式。

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