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פלטפורמה משולבת עבור מיפוי הגנום כולו של כרומטין הברית באמצעות שבב בתפוקה גבוהה-רצף ברקמות הגידול
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Capítulos
- 00:04Título
- 00:31Tissue/Cell Line Processing and Cross-linking
- 01:57Tissue Lysis, Sonication, and Antibody Preparation
- 02:52Chromatin Immunoprecipitation
- 03:40Washing and Reverse Cross-linking of Immunoprecipitated DNA-protein Complexes
- 04:43Purification and Quantification of Precipitated DNA
- 06:00Library Generation Using the NEBNext Ultra II DNA Library Preparation Kit
- 07:53Results: Immunoprecipitation of Tumor Tissues and Cells Using High-throughput ChIP-sequencing
- 09:23Conclusion
