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1Department of Health Sciences,Università del Piemonte Orientale & IRCAD, Novara, Italy, 2Institute of Biomedical Technologies,National Research Council, Segrate, Milan, Italy, 3Institute of Biomedical Technologies,National Research Council, Bari, Italy, 4Department of Life Sciences,University of Trieste, Italy, 5Institute of Genetic and Biomedical Research,National Research Council, Rozzano, Milan, Italy, 6Humanitas Clinical and Research Center, Rozzano, Milan, Italy
Capítulos
- 00:04Título
- 01:19Construction of the ORF Library
- 02:38Preparation of the Filtering Vector
- 03:40Ligation and Transformation
- 06:47pFILTER-ORF Library Validation and Collection
- 09:10Phage Library Deep Sequencing Platform, Index PCR Followed by Qualitative and Quantitative Evaluation of the Sequencing Library
- 10:07Results: Use of a Phagemid Vector to Construct, Characterize and Select a Domainome Library
- 11:23Conclusion
De protokoller er beskrevet tillader opbygning, karakterisering og udvælgelse (mod mål på valg) af en "domainome" bibliotek fra DNA-kilder. Dette opnås ved en forskning rørledning, der kombinerer forskellige teknologier: phage display, en falsning reporter og næste generation sequencing med en web-værktøj til dataanalyse.
