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无输血或强心剂剂的大鼠心肺旁路移植模型

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Imunologia e Infecção

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JoVE Journal Imunologia e Infecção

A Recovery Cardiopulmonary Bypass Model Without Transfusion or Inotropic Agents in Rats

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无输血或强心剂剂的大鼠心肺旁路移植模型

DOI:

10.3791/56986-v

•

09:54 min

•

March 23, 2018

•

Shingo Hirao, Hidetoshi Masumoto, Tatsuya Itonaga, Kenji Minatoya

¹Department of Cardiovascular Surgery, Graduate School of Medicine,Kyoto University

Capítulos

00:05Título
00:41Preparing the CardioPulmonary Bypass (CPB) Circuit
01:56Preparing the Animal for CPB
03:28Cannulation
05:12The CPB Procedure
08:01Results: Physiological Changes with CPB and Histological Analysis
09:22Conclusion

Summary

Tadução automática

在这里, 我们提出一个协议来描述一个简单的恢复心肺旁路模型没有输血或强心剂剂的大鼠。该模型可用于体外循环术后长期多脏器后遗症的研究。

Tags

Keywords: Cardiopulmonary Bypass CPB Recovery Model Transfusion-free Inotropic Agent-free Rodent Model Organ Complications Cardiovascular Cellular Feed Cardiopulmonary Bypass Surgery Priming Solution Membrane Oxygenator Anesthesia Temperature Maintenance ECG Monitoring Cannulation Femoral Artery Jugular Vein

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