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Embryo Microinjection and Transplantation Technique for Nasonia vitripennis Genome Manipulation
JoVE Journal
Genética
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JoVE Journal Genética
Embryo Microinjection and Transplantation Technique for Nasonia vitripennis Genome Manipulation

Embryo Microinjection and Transplantation Technique for Nasonia vitripennis Genome Manipulation

DOI:
10.3791/56990-v

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09:05 min

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December 25, 2017

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, ,
1Department of Entomology and Riverside Center of Disease Vector Research, Institute for Integrative Genome Biology,University of California, Riverside, 2Section of Cell and Developmental Biology, Division of Biological Sciences,University of California, San Diego

Capítulos

  • 00:05Título
  • 00:47N. vitripennis Colony Rearing
  • 01:40Collection and Alignment of N. vitripennis Pre-blastoderm Stage Embryos
  • 04:13Embryo Microinjection
  • 05:58Transplanting Injected G0 N. vitripennis Embryos onto Pre-stung Host
  • 07:15Results: sgRNA and Cas9 Microinjection in N. vitripennis Transplanted Embryos
  • 08:23Conclusion

Summary

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Microinjection of Nasonia vitripennis embryos is an essential method for generating heritable genome modifications. Described here is a detailed procedure for microinjection and transplantation of Nasonia vitripennis embryos, which will greatly facilitate future genome manipulation in this organism.

Tags

Keywords: Embryo MicroinjectionNasonia VitripennisGenome ManipulationVenom ProductionSex DeterminationWasp ColoniesBlowfly PupaeParasitizationEmbryo CollectionEmbryo Alignment SlideMicroinjection Technique

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