/

/

小鼠视网膜色素上皮细胞的原代培养

JoVE Journal
Biologia

É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.

JoVE Journal Biologia

Primary Cell Cultures from the Mouse Retinal Pigment Epithelium

Please note that all translations are automatically generated. Click here for the English version.

小鼠视网膜色素上皮细胞的原代培养

DOI:

10.3791/56997-v

•

09:35 min

•

March 16, 2018

•

Peng Shang, Nadezda A. Stepicheva, Stacey Hose, J. Samuel Zigler, Jr., Debasish Sinha²

¹Department of Ophthalmology,University of Pittsburgh School of Medicine, ²Wilmer Eye Institute,The Johns Hopkins University School of Medicine

Capítulos

00:04Título
00:50Isolating and Preparing Eyeballs for Dissection
03:18Isolating the RPE-choroid-sclera Complex from Eyeballs
05:07Collecting and Culturing Primary RPE Cells from RPE-choroid-sclera Complexes
07:22Results: Characterize Mouse Primary RPE Cultures
08:59Conclusion

Summary

Tadução automática

视网膜色素上皮 (RPE) 是一种多功能的眼睛上皮。在这里, 我们提出了建立从小鼠视网膜色素形成的主要细胞培养的协议。

Tags

Keywords: Primary Cell Culture Mouse Retinal Pigment Epithelium RPE Isolation RPE Cell Culture Euthanasia Eye Dissection Dispase II Laminar Flow Cabinet Growth Medium Microscopy

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

从晚期果蝇蛹的大脑原发性神经文化

小学游离中脑多巴胺鼠类新生儿的细胞培养

原代细胞培养从果蝇原肠胚

人类iPS细胞的微小RNA表达谱，视网膜色素上皮衍生自IPS和胎儿视网膜色素上皮

原发性小鼠视网膜色素上皮细胞的有效解剖与培养

小鼠原代视网膜色素上皮细胞的分离

硝基还原酶/甲硝唑介导的消融和MATLAB平台（RpEGEN）研究斑马鱼视网膜色素上皮的再生

猪视网膜色素上皮细胞原代培养

从人眼高效、一致地生成视网膜色素上皮/脉络膜平面安装，用于组织学分析

基于脂质UNet-机器学习的脂质沉积物使用iPSC衍生的视网膜色素上皮表征和定量脂质沉积物的方法

Read Article