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Primære cellekulturer fra mus retinale Pigment epitel
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Biologia
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JoVE Journal Biologia
Primary Cell Cultures from the Mouse Retinal Pigment Epithelium

Primære cellekulturer fra mus retinale Pigment epitel

DOI:
10.3791/56997-v

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09:35 min

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March 16, 2018

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, , , ,
1Department of Ophthalmology,University of Pittsburgh School of Medicine, 2Wilmer Eye Institute,The Johns Hopkins University School of Medicine

Capítulos

  • 00:04Título
  • 00:50Isolating and Preparing Eyeballs for Dissection
  • 03:18Isolating the RPE-choroid-sclera Complex from Eyeballs
  • 05:07Collecting and Culturing Primary RPE Cells from RPE-choroid-sclera Complexes
  • 07:22Results: Characterize Mouse Primary RPE Cultures
  • 08:59Conclusion

Summary

Tadução automática

Tadução automática

Den retinale pigment epitel (ÅV) er et multi-funktionelle epitel af øjet. Her præsenterer vi en protokol for at oprette primære cellekulturer stammer fra den murine ÅV.

Tags

Primary Cell CultureMouse Retinal Pigment EpitheliumRPE IsolationRPE Cell CultureEuthanasiaEye DissectionDispase IILaminar Flow CabinetGrowth MediumMicroscopy

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