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大范围早期果蝇胚胎的全基因组染色质构象捕获库的生成
JoVE Journal
Genética
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JoVE Journal Genética
Generation of Genome-wide Chromatin Conformation Capture Libraries from Tightly Staged Early Drosophila Embryos
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大范围早期果蝇胚胎的全基因组染色质构象捕获库的生成

DOI:
10.3791/57001-v

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10:35 min

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October 03, 2018

•

,
1Max Planck Institute for Molecular Biomedicine

Capítulos

  • 00:04Título
  • 00:37Embryo Fixation
  • 01:48Embryo Sorting
  • 03:07In Situ Hi-C – Lysis
  • 04:57In Situ Hi-C – Digestion, Overhang Fill-in, and Ligation
  • 06:58DNA Extraction
  • 09:11Results: Analysis of Chromatin Conformation Capture Libraries from Drosophila Embryos
  • 09:58Conclusion

Summary

Tadução automática

Tadução automática

这项工作描述了一个协议, 以产生高分辨率的原位高中图书馆从严格分期预肠果蝇胚胎。

Tags

Keywords: Chromatin Conformation CaptureChromatin ArchitectureGene ExpressionDevelopmentDrosophila EmbryosFormaldehyde FixationCell Cycle StagingEGFP PCNANuclear DensityNuclear CyclesFlash Freezing

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