Journal
/
Imunologia e Infecção
/
Ex Vivo Infection of Human Lymphoid Tissue and Female Genital Mucosa with Human Immunodeficiency Virus 1 and Histoculture
JoVE Journal
Imunologia e Infecção
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Imunologia e Infecção
Ex Vivo Infection of Human Lymphoid Tissue and Female Genital Mucosa with Human Immunodeficiency Virus 1 and Histoculture

Ex Vivo Infection of Human Lymphoid Tissue and Female Genital Mucosa with Human Immunodeficiency Virus 1 and Histoculture

DOI:
10.3791/57013-v

•

11:14 min

•

October 12, 2018

•

, , , ,
1Department of Medicine Solna, Center for Molecular Medicine, Karolinska University Hospital,Karolinska Institutet, 2Section of Intercellular Interactions, Eunice Shriver National Institute of Child Health and Human Development,National Institutes of Health

Capítulos

  • 00:04Título
  • 00:49Preparation of Gelatin Sponges
  • 01:55Dissection and Infection of Tonsillar Tissue with HIV-1
  • 04:13Dissection and Infection of Uterine Cervical Mucosa with HIV-1
  • 07:17Histoculture
  • 08:33Results: Kinetics of HIV-1 Replication in Cervical Mucosa Explants
  • 10:02Conclusion

Summary

Tadução automática

Tadução automática

Infection of human tissues with human immunodeficiency virus (HIV) ex vivo provides a valuable 3D model of virus pathogenesis. Here, we describe a protocol to process and infect tissue specimens from human tonsils and female genital mucosae with HIV-1 and maintain them in culture at the liquid-air interface.

Tags

Keywords: Ex VivoHIVFemale Genital MucosaVirus TransmissionPathogenesisHIV InfectionTonsilsCervixCMT MediumGelatin Sponges

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

check_url/pt/57013?article_type=v

HLA-Ig Based Artificial Antigen Presenting Cells for Efficient ex vivo Expansion of Human CTL
check_url/pt/57013?article_type=v

Isolation of Lymphocytes from Mouse Genital Tract Mucosa
check_url/pt/57013?article_type=v

Assessing the Innate Sensing of HIV-1 Infected CD4+ T Cells by Plasmacytoid Dendritic Cells Using an Ex vivo Co-culture System.
check_url/pt/57013?article_type=v

Lymphocyte Isolation from Human Skin for Phenotypic Analysis and Ex Vivo Cell Culture
check_url/pt/57013?article_type=v

Pairwise Growth Competition Assay for Determining the Replication Fitness of Human Immunodeficiency Viruses
check_url/pt/57013?article_type=v

Ex Vivo Infection of Murine Epidermis with Herpes Simplex Virus Type 1
check_url/pt/57013?article_type=v

Analysis of Lymphocyte Extravasation Using an In Vitro Model of the Human Blood-brain Barrier
check_url/pt/57013?article_type=v

Optical Screening of Novel Bacteria-specific Probes on Ex Vivo Human Lung Tissue by Confocal Laser Endomicroscopy
check_url/pt/57013?article_type=v

Assessment of the Synaptic Interface of Primary Human T Cells from Peripheral Blood and Lymphoid Tissue
check_url/pt/57013?article_type=v

Imaging Cell Interaction in Tracheal Mucosa During Influenza Virus Infection Using Two-photon Intravital Microscopy

Read Article