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JoVE Journal Neuroscience
Single-cell Resolution Fluorescence Live Imaging of Drosophila Circadian Clocks in Larval Brain Culture
 

Single-cell Resolution Fluorescence Live Imaging of Drosophila Circadian Clocks in Larval Brain Culture

Article DOI: 10.3791/57015-v • 07:05 min • January 19th, 2018
January 19th, 2018 •
1, 1
1Department of Genetics and Evolution, University of Geneva

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The goal of this protocol is to establish ex vivo Drosophila larval brain culture optimized to monitor circadian molecular rhythms with long-term fluorescence time-lapse imaging. The application of this method to pharmacological assays is also discussed.

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Keywords: Single-cell Fluorescence Live Imaging Drosophila Circadian Clocks Larval Brain Culture Neurogenetics Gene Expression Fluorescent Probes SAM Medium Thrombin Penicillin Streptomycin Fibrinogen Dissection Forceps DSS Third Instar Larvae Brain Transfer Embedding Procedure
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