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Engineering 'Gyldne' fluorescens af selektiv pres indarbejdelse af ikke-kanoniske aminosyrer og proteinanalyse af massespektrometri og fluorescens
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1Institute of Chemistry L 1, Department of Biocatalysis,Technical University of Berlin, 2Institute of Chemistry PC 14, Department of Bioenergetics,Technical University of Berlin, 3Institute of Chemistry TC 7, Department of Physical Chemistry/Molecular Material Sciences,Technical University of Berlin
Capítulos
- 00:05Título
- 01:46Recombinant Protein Expression
- 04:05Target Protein Purification via Immobilized Metal Ion Affinity Chromatography
- 05:20Intact Protein Mass Analysis by High-performance Liquid Chromatography Coupled to Electrospray Ionization Time-of-flight Mass Spectrometry
- 06:38Fluorescence Lifetime Measurements and Decay-associated Spectra of GdFP
- 07:59Results: Gold Fluorescent Protein Analysis by Mass Spectrometry and Fluorescence
- 10:13Conclusion
Syntetisk biologi giver mulighed for manipulation af proteiner med hidtil uset egenskaber ved hjælp af co-translational indsættelse af ikke-kanoniske aminosyrer. Vi præsenteres her, hvordan en spektralt rød-forskudt variant af en normal god landbrugspraksis-type fluorophore med romanen fluorescens spektroskopiske egenskaber, betegnes "guld" fluorescerende proteiner (GdFP), er produceret i E. coli via selektiv pres indarbejdelse (SPI).
