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小鼠脂肪组织的采集和 RNA 分析处理
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Bioquímica
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JoVE Journal Bioquímica
Mouse Adipose Tissue Collection and Processing for RNA Analysis
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小鼠脂肪组织的采集和 RNA 分析处理

DOI:
10.3791/57026-v

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11:13 min

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January 31, 2018

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, ,
1Centre de Recherche du Centre Hospitalier,Université de Montréal, 2Department of Biochemistry and Molecular Medicine,Université de Montréal, 3Department of Kinesiology,Université de Montréal, 4Montreal Diabetes Research Center

Capítulos

  • 00:05Título
  • 00:52Necropsy and Adipose Tissue Collection from Male Mice
  • 03:38Preparation of Ground White Adipose Tissue for RNA Isolation
  • 04:59RNA Isolation from Ground Adipose Tissue
  • 09:07Results: RNA Analysis of Mouse Adipose Tissue
  • 10:12Conclusion

Summary

Tadução automática

Tadução automática

本文的目的是提出一步一步的程序, 收集不同的白脂肪组织的小鼠, 处理脂肪样本和提取 RNA。

Tags

RNA AnalysisMetabolismBioquímicaBiologia MolecularGene ExpressionSubcutaneous FatPerigonadal FatPerirenal FatLiquid Nitrogen

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