Journal
/
Biologia do Desenvolvimento
/
Proliferation and Differentiation of Murine Myeloid Precursor 32D/G-CSF-R Cells
JoVE Journal
Biologia do Desenvolvimento
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Biologia do Desenvolvimento
Proliferation and Differentiation of Murine Myeloid Precursor 32D/G-CSF-R Cells

Proliferation and Differentiation of Murine Myeloid Precursor 32D/G-CSF-R Cells

DOI:
10.3791/57033-v

•

10:21 min

•

February 21, 2018

•

, , ,
1Department of Hemato-Oncology,Institute of Molecular Genetics of the ASCR, 2Childhood Leukaemia Investigation Prague, Department of Paediatric Haematology and Oncology, 2nd Faculty of Medicine,Charles University

Capítulos

  • 00:04Título
  • 00:58Media Preparation
  • 01:24Production of Retrovirus
  • 03:28Tritation of Virus Containing a Puromycin Reporter
  • 05:04Transduction of 32D/G-CSF-R Cells
  • 06:1032D/G-CSF-R Cell Differentiation Assay
  • 07:32Results: Study of Proliferation and Differentiation of 32D/G-CSF-R Cells in Interleukin-3 and Granulocyte Colony Stimulating Factor Containing Media
  • 09:21Conclusion

Summary

Tadução automática

Tadução automática

Here detailed protocols for culturing the murine myeloid precursor 32D/G-CSF-R cell line, performing viral infections, and carrying out proliferation and differentiation assays are presented. This cell line is suitable for studying myeloid cell development, and the role of genes of interest in myeloid cell growth and neutrophilic differentiation.

Tags

Keywords: Myeloid Precursor Cells32D-G-CSF-R CellsProliferationDifferentiationGenetic ModificationRetroviral TransductionBosc23 CellsRPMI-1640 MediumInterleukin-3MSCV Retroviral ConstructPCL Echo Packaging VectorPolyethylenimineDMEM MediumFetal Bovine SerumVirus ProductionVirus Titration

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

check_url/pt/57033?article_type=v

Production and Use of Lentivirus to Selectively Transduce Primary Oligodendrocyte Precursor Cells for In Vitro Myelination Assays
check_url/pt/57033?article_type=v

3D Organotypic Co-culture Model Supporting Medullary Thymic Epithelial Cell Proliferation, Differentiation and Promiscuous Gene Expression
check_url/pt/57033?article_type=v

Epigenetic Regulation of Cardiac Differentiation of Embryonic Stem Cells and Tissues
check_url/pt/57033?article_type=v

Preparation and Culture of Myogenic Precursor Cells/Primary Myoblasts from Skeletal Muscle of Adult and Aged Humans
check_url/pt/57033?article_type=v

In Vitro Differentiation of Human Mesenchymal Stem Cells into Functional Cardiomyocyte-like Cells
check_url/pt/57033?article_type=v

Rapid Detection of Neurodevelopmental Phenotypes in Human Neural Precursor Cells (NPCs)
check_url/pt/57033?article_type=v

Computational Analysis of the Caenorhabditis elegans Germline to Study the Distribution of Nuclei, Proteins, and the Cytoskeleton
check_url/pt/57033?article_type=v

Derivation and Differentiation of Canine Ovarian Mesenchymal Stem Cells
check_url/pt/57033?article_type=v

Efficient Differentiation of Human Pluripotent Stem Cells into Liver Cells
check_url/pt/57033?article_type=v

In Vitro Generation of Somite Derivatives from Human Induced Pluripotent Stem Cells

Read Article