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用于在大容量中查找特定细胞目标的串行剖面的多模式分层成像
JoVE Journal
Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Multimodal Hierarchical Imaging of Serial Sections for Finding Specific Cellular Targets within Large Volumes
DOI:

11:19 min

March 20, 2018

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Capítulos

  • 00:04Título
  • 01:16Ultramicrotome and Sample Setup
  • 03:04Ultramicrotome Sectioning
  • 04:21Hierarchical Imaging in the SEM
  • 07:33Registration of the SEM Image Stack
  • 08:39Results: Multimodal Hierarchical Imaging of Serial Sections of Plant Roots
  • 10:06Conclusion

Summary

Tadução automática

本协议以扫描电镜 (SEM) 为目标, 以纳米尺度分辨率成像组织中的特定细胞为靶点。从树脂嵌入的生物材料中, 大量的系列部分首先在光学显微镜中被成像, 以识别目标, 然后以分层的方式在 SEM 中。

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