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Beoordeling van extracellulaire vesikel functie tijdens Malaria infectie
JoVE Journal
Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Evaluation of Extracellular Vesicle Function During Malaria Infection
DOI:

13:45 min

February 14, 2018

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Capítulos

  • 00:04Título
  • 00:49Fluorescence Labeling of Extracellular Vesicles
  • 03:05Visualize Uptake of Extracellular Vesicles by Confocal Microscopy
  • 06:18Endothelial Cell Permeability
  • 07:36Determine Puromycin Sensitivity
  • 08:59Transduction of Endothelial Cells with Lentiviral Vector
  • 10:53Results: Use of Confocal Microscopy and Diffusion Capacity of Fluorochrome Dextran to Study the Interaction Between the Extracellular Vesicles and Endothelial Cells
  • 12:50Conclusion

Summary

Tadução automática

In dit werk beschrijven we protocollen voor het onderzoek naar de rol van extracellulaire blaasjes (EVs) uitgebracht door Plasmodium falciparum geïnfecteerd erytrocyten. In het bijzonder richten we ons op de interacties van EVW met endotheliale cellen.

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