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Biologia do Desenvolvimento

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老年人刷膜的体外模型对视网膜色素上皮细胞的培养

JoVE Journal
Biologia do Desenvolvimento

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JoVE Journal Biologia do Desenvolvimento

Culturing of Retinal Pigment Epithelial Cells on an Ex Vivo Model of Aged Human Bruch’s Membrane

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老年人刷膜的体外模型对视网膜色素上皮细胞的培养

DOI:

10.3791/57084-v

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08:32 min

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April 12, 2018

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Hui Cai, Jie Gong, Lucian V. Del Priore, Tongalp H. Tezel, Mark A. Fields

¹Department of Ophthalmology and Visual Science,Yale School of Medicine, ²Edward S. Harkness Eye Institute,Columbia University School of Medicine

Capítulos

00:05Título
01:04Harvesting of Human Bruch’s Membrane Explants
04:53Culturing of Retinal Pigment Epithelial (RPE) Cells on Human Bruch’s Membrane Explants
06:36Results: Culturing RPE Cells on Aged Bruch’s Membranes
07:19Conclusion

Summary

Tadução automática

本协议的目的是展示视网膜色素上皮 (RPE) 细胞在老年和/或患病的人刷膜的培养。该方法适用于细胞外基质上皮细胞行为的研究。

Tags

Keywords: Retinal Pigment Epithelial Cells Bruch’s Membrane Ex Vivo Model Aged Human AMD Etiology Proteomics RPE Cell Culture Sclera Vitreous Retina Optic Nerve Ammonium Hydroxide DPBS PTFE Membrane

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