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核素荧光报告基因成像在啮齿动物肿瘤模型中跟踪肿瘤进展

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JoVE Journal Pesquisa do Câncer

Radionuclide-fluorescence Reporter Gene Imaging to Track Tumor Progression in Rodent Tumor Models

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核素荧光报告基因成像在啮齿动物肿瘤模型中跟踪肿瘤进展

DOI:

10.3791/57088-v

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10:04 min

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March 13, 2018

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Alessia Volpe, Francis Man, Lindsay Lim, Alex Khoshnevisan, Julia Blower, Philip J. Blower, Gilbert O. Fruhwirth

¹Department of Imaging Chemistry and Biology, School of Biomedical Engineering and Imaging Sciences,King’s College London

Capítulos

00:04Título
01:40Engineering and Characterization of Cancer Cells to Express the Radionuclide-fluorescence Fusion Reporter NIS-FP and [¹⁸F]BF₄ &#45 Synthesis
04:04In Vivo Imaging of NIS-FP Expressing Cells by nanoPET/CT
05:40Ex Vivo Analyses
06:33Results: Radiotracer Uptake in Cell Lines is Due to Specific NIS-FP Expression
08:24Conclusion

Summary

Tadução automática

我们描述了一个协议, 为临床前在体内跟踪肿瘤转移。它的基础上的放射性核素荧光记者结合碘化钠转运体, 检测到非侵入性 [18 F] 氟硼酸盐-PET, 和荧光蛋白, 简化了前体确认. 该方法适用于肿瘤生物学以外的临床前体内细胞追踪。

Tags

Keywords: Radionuclide Fluorescence Reporter Gene Tumor Progression Rodent Tumor Models PET In Vivo Imaging Cell Tracking Automated Synthesis Preclinical Testing Cancer Biology Therapeutic Development

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