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Isolation and Culture of Rodent Microglia to Promote a Dynamic Ramified Morphology in Serum-free Medium
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Neurociência
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JoVE Journal Neurociência
Isolation and Culture of Rodent Microglia to Promote a Dynamic Ramified Morphology in Serum-free Medium

Isolation and Culture of Rodent Microglia to Promote a Dynamic Ramified Morphology in Serum-free Medium

DOI:
10.3791/57122-v

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12:00 min

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March 09, 2018

•

,
1Department of Neurobiology,Stanford University

Capítulos

  • 00:04Título
  • 00:41Tissue Collection and Mechanical Dissociation
  • 02:52Myelin Removal
  • 04:07Immunopanning
  • 07:56Spot Coating Tissue Culture/Coverslips
  • 09:22Results: Defined-medium Microglia Cultures Show Ramified Morphology with Dynamic Processes
  • 11:22Conclusion

Summary

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Efforts to understand microglial function in detail have been hindered by the lack of microglial culture models that recapitulate the properties of mature in vivo microglia. This protocol describes an isolation and culture approach designed to maintain robust survival of highly ramified mature rat microglia under defined-medium conditions.

Tags

Keywords: MicrogliaIsolationCultureSerum-freeRodentMorphologyCNSDounce HomogenizerMSBDPBS

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