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鼠小胶质细胞的分离培养促进无血清培养基动态分支形态学的研究

JoVE Journal
Neurociência

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JoVE Journal Neurociência

Isolation and Culture of Rodent Microglia to Promote a Dynamic Ramified Morphology in Serum-free Medium

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鼠小胶质细胞的分离培养促进无血清培养基动态分支形态学的研究

DOI:

10.3791/57122-v

•

12:00 min

•

March 09, 2018

•

Hannah Y. Collins, Christopher J. Bohlen

¹Department of Neurobiology,Stanford University

Capítulos

00:04Título
00:41Tissue Collection and Mechanical Dissociation
02:52Myelin Removal
04:07Immunopanning
07:56Spot Coating Tissue Culture/Coverslips
09:22Results: Defined-medium Microglia Cultures Show Ramified Morphology with Dynamic Processes
11:22Conclusion

Summary

Tadução automática

由于缺乏对成熟的体内小胶质细胞的特性进行重述的胶质文化模型, 胶质的详细理解的努力受到了阻碍。该协议描述了一种隔离和培养方法, 旨在维持高分支成熟大鼠小胶质细胞在确定培养基条件下的健壮生存。

Tags

Microglia Isolation Culture Serum-free Rodent Morphology CNS Dounce Homogenizer MSB DPBS

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