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Imunologia e Infecção
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Histological Quantification to Determine Lung Fungal Burden in Experimental Aspergillosis
JoVE Journal
Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Histological Quantification to Determine Lung Fungal Burden in Experimental Aspergillosis

Histological Quantification to Determine Lung Fungal Burden in Experimental Aspergillosis

DOI:
10.3791/57155-v

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09:52 min

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March 09, 2018

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, , , ,
1Department of Microbiology and Immunology,Indiana University School of Medicine – Terre Haute

Capítulos

  • 00:05Título
  • 01:04Aspergillus Fumigatus Conidia Infection
  • 03:09Mouse Lung Harvest and Preservation
  • 05:09Light Microscopy Imaging and Fungal Burden Calculation
  • 07:26Results: Representative Survival, Fungal Burden, and Fungal Clearance in a Mouse Model of Invasive Aspergillus Infection
  • 08:53Conclusion

Summary

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Here we describe a protocol to determine pulmonary fungal burden in mice with invasive aspergillosis by quantification of Gomori's modified methanamine silver staining in histological sections. Use of this method resulted in comparable results with less animals compared to assessment of fungal burden by quantitative PCR of lung fungal DNA.

Tags

Histological QuantificationLung Fungal BurdenInvasive AspergillosisExperimental AspergillosisQuantitative PCRNeutrophil DepletionAspergillus FumigatusConidiaIntranasal InfectionHistological Analysis

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