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洗剤に敏感な膜タンパク質相互作用の検出
JoVE Journal
Bioquímica
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JoVE Journal Bioquímica
Detection of Detergent-sensitive Interactions Between Membrane Proteins
DOI:

10:09 min

March 07, 2018

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Capítulos

  • 00:04Título
  • 01:05Handling of the Peptide and Cells
  • 03:02Binding of His-tagged Proteins to the HisPur Cobalt Beads
  • 04:39Binding of the Peptide to the His-tagged Protein Immobilized on the Beads
  • 05:59Elution from Cobalt Beads
  • 07:07Electrophoresis, Western Blotting, and Analysis of Results
  • 07:58Results: Use of Immunoblot Assay to Study the Interaction between Sortilin Immobilized on Cobalt Beads and myc-tagged Peptide
  • 09:18Conclusion

Summary

Tadução automática

洗剤に敏感な例として並べ替え受容体、ソーティリン、GLUT4 グルコース輸送体タンパク質の最初の内腔のループに結合を用いた膜タンパク質相互作用の検出のためのプロトコルについて述べる。

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