/

Pesquisa do Câncer

/

一种确定环境富集对小鼠结肠肿瘤模型结肠菌群生物多样性影响的方法

JoVE Journal
Pesquisa do Câncer

É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.

JoVE Journal Pesquisa do Câncer

A Method to Define the Effects of Environmental Enrichment on Colon Microbiome Biodiversity in a Mouse Colon Tumor Model

Please note that all translations are automatically generated. Click here for the English version.

一种确定环境富集对小鼠结肠肿瘤模型结肠菌群生物多样性影响的方法

DOI:

10.3791/57182-v

•

08:14 min

•

February 28, 2018

•

Andrew K. Fuller², Benjamin D. Bice², Ashlee R. Venancio², Olivia M. Crowley², Ambur M. Staab², Stephanie J. Georges², Julio R. Hidalgo², Annika V. Warncke², Melinda L. Angus-Hill²

¹Division of Gastroenterology, Hepatology, and Nutrition, Department of Internal Medicine,University of Utah, ²Huntsman Cancer Institute,University of Utah

Capítulos

00:04Título
00:50Environmentally Enriched Cage Set Up
02:36Stool Collection at 16 Weeks of Age
03:53Processing gDNA from the Stool
06:00Results: The Effect of Enhanced Environments on Microbiome Biodiversity
07:28Conclusion

Summary

Tadução automática

环境富集 (EE) 是一种动物住房环境, 用于揭示生活方式、压力和疾病之间联系的机制。该协议描述了一个使用结肠肿瘤发生的小鼠模型和 EE 的过程, 专门定义可能影响动物死亡率的微生物群落生物多样性的变化。

Tags

Environmental Enrichment Colon Microbiome Colon Tumor Model Microbiome Normalization Experimental Reproducibility Animal Stress Reduction Breeding Pup Rearing Stool Collection

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

该<em>果蝇</em>成虫盘瘤模型：基因的表达与肿瘤侵袭性的可视化和定量遗传马赛克

结肠-26癌荷瘤小鼠为癌症恶病质的研究模型

人地幔细胞淋巴瘤移植小鼠模型中肿瘤植入的研究

骨肉瘤前小鼠模型对肿瘤与间充质干细胞细胞外囊泡介导通信的定义

用基因编码荧光传感器评估毒理学氧化应激的成像方法

自体胰腺癌小鼠模型研究不可逆电穿孔的影响

淋巴结慢病毒北疆-转基因 Organoids 培养的大鼠结肠肿瘤的高灵敏度检测

数字聚合酶链反应法测定零星家族性腺瘤性息肉病患者的基因变异

患者衍生的人类泌尿细胞癌和结肠直肠癌肿瘤生长和自发转移的正交异种移植模型

人类结肠癌细胞DNA损伤与修复福西的免疫荧光成像

Read Article