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微生物菌落生物膜的石蜡嵌入和薄切片显微分析

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Imunologia e Infecção

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JoVE Journal Imunologia e Infecção

Paraffin Embedding and Thin Sectioning of Microbial Colony Biofilms for Microscopic Analysis

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微生物菌落生物膜的石蜡嵌入和薄切片显微分析

DOI:

10.3791/57196-v

•

08:52 min

•

March 23, 2018

•

William C. Cornell, Chase J. Morgan, Leslie Koyama², Hassan Sakhtah, Jennifer H. Mansfield, Lars E.P. Dietrich

¹Department of Biological Sciences,Columbia University, ²Department of Biology, Barnard College,Columbia University

Capítulos

00:04Título
00:49Growth of Pseudomonas aeruginosa Colony Biofilms and Overlaying Colonies with Agar
02:33Fixation and Sample Processing
03:02Sample Embedding and Sectioning
05:23Heat Fixing, Rehydration, and Mounting
06:48Results: Microscopic Analysis of Microbial Colony Biofilms
08:01Conclusion

Summary

Tadução automática

我们描述了微生物菌落生物膜的固定、石蜡嵌入和薄切片技术。在制备的样品中, 生物膜子结构和记者的表达模式可以通过显微技术进行可视化。

Tags

Keywords: Paraffin Embedding Thin Sectioning Microbial Colony Biofilms Microscopic Analysis Gene Expression Fluorescent Protein Biofilm Architecture Exopolymeric Substances Agar Tryptone Solution P. Aeruginosa Colony Biofilms Embedding Cassette Fixative PBS Microscopy

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