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En Flow flowcytometri-baserede analyse til at identificere stoffer, der forstyrrer bindingen af Fluorescently-mærket CXC Chemokine Ligand 12 til CXC Chemokine Receptor 4
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Biologia
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JoVE Journal Biologia
A Flow Cytometry-based Assay to Identify Compounds That Disrupt Binding of Fluorescently-labeled CXC Chemokine Ligand 12 to CXC Chemokine Receptor 4

En Flow flowcytometri-baserede analyse til at identificere stoffer, der forstyrrer bindingen af Fluorescently-mærket CXC Chemokine Ligand 12 til CXC Chemokine Receptor 4

DOI:
10.3791/57271-v

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06:56 min

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March 10, 2018

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, , , ,
1Laboratory of Virology and Chemotherapy, Department of Microbiology and Immunology, Rega Institute for Medical Research,KU Leuven

Capítulos

  • 00:04Título
  • 00:54Competition Binding Assay
  • 03:00Analysis of the Samples by Flow Cytometry
  • 05:35Results: Evaluation and Analysis of Active and Inactive Compounds in the Competition Binding Assay
  • 06:35Conclusion

Summary

Tadução automática

Tadução automática

En flow flowcytometri-baserede cellulære bindende assay er beskrevet, som bruges primært som en screeningsværktøj til at identificere stoffer, der hæmmer bindingen af en fluorescently mærket CXC chemokine ligand 12 (CXCL12) til CXC chemokine receptor 4 (CXCR4).

Tags

Flow CytometryBinding AssayCXCR4CXCL12G-protein Coupled ReceptorFluorescent LigandCompetition BindingDrug Discovery

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