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在斑马鱼胚胎中通过细胞 Photoconversion 的心内膜组织运动后

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Biologia do Desenvolvimento

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JoVE Journal Biologia do Desenvolvimento

Following Endocardial Tissue Movements via Cell Photoconversion in the Zebrafish Embryo

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在斑马鱼胚胎中通过细胞 Photoconversion 的心内膜组织运动后

DOI:

10.3791/57290-v

•

09:38 min

•

February 20, 2018

•

Renee Wei-Yan Chow*^1,2,3,4, Paola Lamperti*^1,2,3,4, Emily Steed^2,3,4, Francesco Boselli^2,3,4, Julien Vermot^2,3,4

¹Institut de Génétique et de Biologie Moléculaire et Cellulaire, ²UMR7104,Centre National de la Recherche Scientifique, ³U964,Institut National de la Santé et de la Recherche Médicale, ⁴Université de Strasbourg

Capítulos

00:04Título
00:53Preparing Molds and Mounting Agarose and Embedding Embryos
03:31Photoconversion
05:12Unmounting Photoconverted Embryos
06:20Imaging Photoconverted Cells at Later Embryonic Stages
08:05Results: Photoconversion of Kaede Fluorescent Protein in Zebrafish Embryonic Endocardial Cells
09:01Conclusion

Summary

Tadução automática

该协议描述了在活斑马鱼胚胎的心内膜细胞中 photoconversion 枫荧光蛋白的方法, 能够在房室管和房室心脏瓣膜发育过程中跟踪心内膜细胞。.

Tags

Cell Photoconversion Zebrafish Embryo Atrioventricular Canal Heart Valve Formation Cardiac Development Mutants Time-lapse Microscopy Agarose Mounting Medium Low Melting Point Agarose Tricaine BDM

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