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在秀丽线虫中对倍体的操控
JoVE Journal
Genética
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JoVE Journal Genética
Manipulation of Ploidy in Caenorhabditis elegans
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在秀丽线虫中对倍体的操控

DOI:
10.3791/57296-v

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07:54 min

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March 15, 2018

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, , , ,
1Brooklyn College, Biology Department,City University of New York, 2The Graduate Center, Biology Department,City University of New York, 3Advanced Science Research Center,City University of New York

Capítulos

  • 00:04Título
  • 01:25Setting Up rec-8 RNAi (Day 1 – 3 in Figure 2)
  • 02:32Generating and Isolating Tetraploids (Day 4 – 16 in Figure 2)
  • 03:57Verifying Tetraploid Strains using DAPI Staining
  • 05:56Results: Generation of Tetraploid C. elegans Strains
  • 07:11Conclusion

Summary

Tadução automática

Tadução automática

这种方法允许生成二、四倍体杂交和无菌的秀丽线虫从任何双倍菌株。这种方法产生的多倍体菌株用于研究减数分裂前期的染色体相互作用, 这一方法对于研究细胞、发育、进化和肿瘤生物学等重要的基本问题是有用的。

Tags

TetraploidPloidyCaenorhabditis ElegansRec-8RNAiPolyploidizationGene DosageGenome InstabilitySpeciationDiploidSpermatocytesOocytesE. ColiIPTGAmpicillinLBHermaphroditesL4 Stage

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