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Miniaturized Sample Preparation for Transmission Electron Microscopy
JoVE Journal
Biologia
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JoVE Journal Biologia
Miniaturized Sample Preparation for Transmission Electron Microscopy

Miniaturized Sample Preparation for Transmission Electron Microscopy

DOI:
10.3791/57310-v

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09:04 min

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July 27, 2018

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, , , , , , , , , ,
1Center for Cellular Imaging and NanoAnalytics (C-CINA), Biozentrum,University of Basel, 2Swiss Nanoscience Institute,University of Basel, 3BioEM lab, Biozentrum,University of Basel

Capítulos

  • 00:04Título
  • 00:46Cryo Grid Preparation
  • 02:42Grid Preparation Macro
  • 03:56Single-cell Lysate Preparation
  • 05:54Cell Lysis Macro
  • 07:32Results: Imaging of Membrane Structures Using the Cryowriter
  • 08:32Conclusion

Summary

Tadução automática

Tadução automática

An instrument and methods for the preparation of nanoliter-sized sample volumes for transmission electron microscopy is presented. No paper-blotting steps are required, thus avoiding the detrimental consequences this can have for proteins, significantly reducing sample loss and enabling the analysis of single cell lysate for visual proteomics.

Tags

Keywords: Miniaturized Sample PreparationTransmission Electron MicroscopySingle Cell AnalysisVisual ProteomicsProtein-related DiseaseCryo-EMSample PreparationEthane CupCryogenLiquid NitrogenEthane GasEM GridMicrocapillarySystem Liquid

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