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Menselijke pluripotente stamcel cultuur op Polyvinyl Alcohol-Co-itacon zuur Hydrogels met verschillende stijfheid onder voorwaarden Xeno-gratis
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1Department of Chemical and Materials Engineering,National Central University, 2Department of Botany and Microbiology,King Saud University, 3Cathay Medical Research Institute,Cathay General Hospital, 4Graduate Institute of Systems Biology and Bioinformatics,National Central University, 5Department of Medical Microbiology and Parasitology,Universiti Putra Malaysia, 6Department of Internal Medicine,Taiwan Landseed Hospital, 7Department of Zoology,Bharathiar University, 8Thiruvalluvar University
Capítulos
- 00:04Título
- 01:00Polyvinyl Alcohol-Co-Itaconic Acid (P-IA) Hydrogel Dish Preparation and Grafting
- 05:02Passaging of Undifferentiated Human Embryonic Stem (ES) and Human Induced Pluripotent Stem (iPS) Cells on Hydrogels
- 06:37Characterization of the Expression of Pluripotency Proteins by Immunostaining
- 08:49Results: Human ES and iPS Pluripotency on Conventional Dishes and Hydrogel Dishes
- 10:19Conclusion
Een protocol is bedoeld om te bereiden polyvinyl alcohol-co-itacon zuur hydrogels met verschillende stijfheid, die waren geënt met en zonder oligopeptides, te onderzoeken van het effect van de stijfheid van biomaterialen op de differentiatie en de verspreiding van de cellen van de stam. De stijfheid van de hydrogels werd gecontroleerd door het crosslinking tijd.
