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Imunologia e Infecção
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Bile Salt-induced Biofilm Formation in Enteric Pathogens: Techniques for Identification and Quantification
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Bile Salt-induced Biofilm Formation in Enteric Pathogens: Techniques for Identification and Quantification

Bile Salt-induced Biofilm Formation in Enteric Pathogens: Techniques for Identification and Quantification

DOI:
10.3791/57322-v

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10:05 min

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May 06, 2018

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,
1Mucosal Immunology and Biology Research Center, Division of Pediatric Gastroenterology and Nutrition,Massachusetts General Hospital, 2Department of Pediatrics,Harvard Medical School

Capítulos

  • 00:04Título
  • 01:08Solid-phase Adherence Assay
  • 03:55Extracellular Polymeric Substance Matrix Detection
  • 05:20Dispersion Assay
  • 07:07Results: Use of Solid-phase Adherence and Extracellular Polymeric Substance Matrix Formation to Detect Biofilm Formation and Dispersion
  • 08:43Conclusion

Summary

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This protocol enables the reader to analyze bile salt-induced biofilm formation in enteric pathogens using a multifaceted approach to capture the dynamic nature of bacterial biofilms by assessing adherence, extracellular polymeric substance matrix formation, and dispersion.

Tags

Bile SaltsBiofilm FormationEnteric PathogensBacterial PathogenesisShigella FlexneriCrystal Violet AssayQuantification96-well PlateStatic IncubationPBS Washing

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