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胆汁盐诱导肠道病原菌生物膜形成的鉴别和定量技术

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Imunologia e Infecção

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JoVE Journal Imunologia e Infecção

Bile Salt-induced Biofilm Formation in Enteric Pathogens: Techniques for Identification and Quantification

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胆汁盐诱导肠道病原菌生物膜形成的鉴别和定量技术

DOI:

10.3791/57322-v

•

10:05 min

•

May 06, 2018

•

Kourtney P. Nickerson², Christina S. Faherty²

¹Mucosal Immunology and Biology Research Center, Division of Pediatric Gastroenterology and Nutrition,Massachusetts General Hospital, ²Department of Pediatrics,Harvard Medical School

Capítulos

00:04Título
01:08Solid-phase Adherence Assay
03:55Extracellular Polymeric Substance Matrix Detection
05:20Dispersion Assay
07:07Results: Use of Solid-phase Adherence and Extracellular Polymeric Substance Matrix Formation to Detect Biofilm Formation and Dispersion
08:43Conclusion

Summary

Tadução automática

该协议使读者能够分析胆汁盐诱导的肠道致病菌的生物膜形成, 采用多层面的方法来通过评估附着体、胞外高分子物质基质形成来捕获细菌生物膜的动态特性,和分散。

Tags

Bile Salts Biofilm Formation Enteric Pathogens Bacterial Pathogenesis Shigella Flexneri Crystal Violet Assay Quantification 96-well Plate Static Incubation PBS Washing

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