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Diabetogenic पर्यावरणीय रसायनों के लिए एक Murine अग्नाशय टाप सेल आधारित स्क्रीनिंग
JoVE Journal
Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
A Murine Pancreatic Islet Cell-based Screening for Diabetogenic Environmental Chemicals
DOI:

07:39 min

June 25, 2018

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Capítulos

  • 00:04Título
  • 00:49Pancreas Perfusion and Removal
  • 01:43Pancreas Digestion and Purification of Islets
  • 03:30Plating and Treatment of Islet Cells
  • 04:23ROS Staining and Measurement
  • 05:28Results: Xenobiotic Treatment-induced Reactive Oxygen Species and Inhibition of Insulin Secretion in Isolated Pancreatic Islet Cells
  • 06:39Conclusion

Summary

Tadução automática

यहां हम xenobiotics द्वारा ROS प्रेरण स्क्रीनिंग के लिए माउस अग्नाशय टाप कोशिकाओं को अलग करने के लिए एक प्रोटोकॉल प्रस्तुत क्रम में संभावित diabetogenic xenobiotic रसायनों की पहचान करने के लिए ।

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