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1Department of Molecular Cell Biology,University of California, Berkeley, 2Howard Hughes Medical Institute,University of California, Berkeley, 3Innovative Genomics Institute,University of California, Berkeley, 4Biomedical Sciences Graduate Program,University of California, San Francisco, 5Department of Microbiology and Immunology,University of California, San Francisco, 6Diabetes Center,University of California, San Francisco, 7Chan Zuckerberg Biohub, 8Department of Medicine,University of California, San Francisco, 9UCSF Helen Diller Family Comprehensive Cancer Center,University of California, San Francisco, 10Department of Integrative Biology,University of California, Berkeley
Capítulos
- 00:04Título
- 01:09RNP Preparation for Primary Cell Editing, Hematopoietic Stem/Progenitor Cell (HSPC) Preparation, and RNP Electroporation
- 02:46C. elegans Gonad Microinjection with RNPs and Post-injection Care
- 05:17P. hawaiensis Embryo Microinjection with RNPs and Post-injection Care
- 08:05Results: Evaluation of Genome Editing Outcomes
- 09:28Conclusion
Unter Verwendung einer vormontierten Cas9 ist Ribonucleoprotein Komplex (RNP) eine leistungsfähige Methode für präzise und effiziente Genom-Bearbeitung. Hier heben wir ihre Nützlichkeit in einem breiten Spektrum von Zellen und Organismen, einschließlich der primären humanen Zellen und sowohl klassische und neue Modellorganismen.
