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尼罗红与油红 O 染色法测定秀丽线虫的脂质丰度及脂质分布评价

JoVE Journal
Biologia

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JoVE Journal Biologia

Quantification of Lipid Abundance and Evaluation of Lipid Distribution in Caenorhabditis elegans by Nile Red and Oil Red O Staining

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尼罗红与油红 O 染色法测定秀丽线虫的脂质丰度及脂质分布评价

DOI:

10.3791/57352-v

•

07:46 min

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March 05, 2018

•

Wilber Escorcia, Dana L. Ruter^2,3, James Nhan², Sean P. Curran²

¹Leonard Davis School of Gerontology,University of Southern California, ²Molecular & Computational Biology Section,University of Southern California, ³Integrative Program for Biological & Genome Sciences,University of North Carolina at Chapel Hill

Capítulos

00:05Título
00:34Nile Red Staining of Lipids
03:42Oil Red O Staining of Lipids
05:28Results: Lipid Quantification and Distribution in C. elegans by Nile Red and Oil Red O Staining
06:53Conclusion

Summary

Tadução automática

固定秀丽线虫的尼罗河红色染色是一种定量测量中性脂质矿床的方法, 而油红色 O 染色有助于定性评价组织间脂质的分布。

Tags

Keywords: Lipid Abundance Lipid Distribution Caenorhabditis Elegans Nile Red Oil Red O Lipid Metabolism Lipid Homeostasis OP50 E.coli PBST Isopropanol NR Working Solution FITC GFP Channel

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