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Construction of Synthetic Phage Displayed Fab Library with Tailored Diversity
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Construction of Synthetic Phage Displayed Fab Library with Tailored Diversity

Construction of Synthetic Phage Displayed Fab Library with Tailored Diversity

DOI:
10.3791/57357-v

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12:31 min

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May 01, 2018

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, , , , ,
1Laboratory of Antibody Engineering, Shanghai Institute for Advanced Immunochemical Studies,ShanghaiTech University, 2Banting and Best Department of Medical Research, Terrence Donnelly Center for Cellular and Biomolecular Research,University of Toronto, 3Department of Molecular Genetics, Terrence Donnelly Center for Cellular and Biomolecular Research,University of Toronto

Capítulos

  • 00:04Título
  • 01:18Electroporation and Calculation of the Library Size
  • 06:02Quality Assessment by Protein A/L Direct Binding ELISA Assay and Sequencing
  • 09:43Results: Conversion of dU-ssDNA to CCC-DNA and Phage Titration for Calculation of Library Size
  • 11:07Conclusion

Summary

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Tadução automática

This protocol describes a detailed procedure for the construction of a phage-displayed synthetic antibody library with tailored diversity. Synthetic antibodies have broad applications from basic research to disease diagnostics and therapeutics.

Tags

Keywords: Synthetic Phage DisplayFab LibraryAntibody Library ConstructionAntibody DiversityMonoclonal Antibody DevelopmentPhage Display TechnologyKunkel MutagenesisElectroporationDiversity EvaluationELISA

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