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Micromanipulation of Chromosomes in Insect Spermatocytes
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Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Micromanipulation of Chromosomes in Insect Spermatocytes

Micromanipulation of Chromosomes in Insect Spermatocytes

DOI:
10.3791/57359-v

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05:45 min

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October 22, 2018

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, , , ,
1Program in Cell Biology/Biochemistry,Bucknell University, 2Department of Electrical and Computer Engineering,Bucknell University, 3Biology Department,Bucknell University

Capítulos

  • 00:04Título
  • 00:34Preparation of Primary Insect Spermatocyte Cell Culture for Micromanipulation
  • 01:11Spermatocyte Culture Preparation for Viewing on Microscope
  • 02:03Production of the Microneedle
  • 02:42Positioning the Micromanipulator
  • 04:00Micromanipulation of Chromosomes
  • 04:43Results: Chromosomes Can be Detached from Spindle in Prophase, Metaphase, and Anaphase
  • 05:07Conclusion

Summary

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In this protocol, we describe the selection and preparation of appropriate cells for micromanipulation and the use of a piezoelectric micromanipulator to reposition chromosomes within those cells.

Tags

Keywords: MicromanipulationChromosomesInsect SpermatocytesCell BiologySpindle CheckpointSpindle AttachmentMiosisNonlethal TechniqueTensionGlass SlideDissection WellHalocarbon OilTestesForcepsMicroscopeMicroneedleMicromanipulator

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