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Electrically Conductive Scaffold to Modulate and Deliver Stem Cells
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JoVE Journal Bioengenharia
Electrically Conductive Scaffold to Modulate and Deliver Stem Cells

Electrically Conductive Scaffold to Modulate and Deliver Stem Cells

DOI:
10.3791/57367-v

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05:49 min

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April 13, 2018

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, , , ,
1Department of Neurology and Neurological Sciences,Stanford University School of Medicine, 2Stanford Stroke Center and Stanford University School of Medicine

Capítulos

  • 00:04Título
  • 00:52Fabrication of the In Vitro Electrical Stimulation Chamber
  • 02:42In Vivo Polypyrrole Implantation
  • 04:15Results: Electrical Stimulation of hNPCs
  • 05:00Conclusion

Summary

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This protocol describes fabrication of a cell culture system to allow seeding of stem cells on a conductive polymer scaffold for in vitro electrical stimulation and subsequent in vivo implantation of the stem cell-seeded scaffold using a minimally invasive technique.

Tags

Electrical StimulationStem CellsConductive Polymer ScaffoldCell DeliveryStroke ResearchCell OptimizationCraniectomyPDMSPolypyrroleSilver PasteEpoxyElectrical FieldCell ImplantationAmpicillinAnesthesiaCraniectomy

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