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高通量 SiRNA 筛选氯化苦和氟化氢引起的角膜上皮细胞损伤
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Biologia
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JoVE Journal Biologia
High Throughput SiRNA Screening for Chloropicrin and Hydrogen Fluoride-Induced Cornea Epithelial Cell Injury
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高通量 SiRNA 筛选氯化苦和氟化氢引起的角膜上皮细胞损伤

DOI:
10.3791/57372-v

•

14:20 min

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June 16, 2018

•

, , ,
1US Army Medical Research Institute of Chemical Defense

Capítulos

  • 00:00Título
  • 00:23Plate cells for experimentation
  • 02:02Transfection
  • 04:37Positive control addition
  • 05:41HF exposure of cultured cells
  • 07:32Sample collection and cell viability assay
  • 10:04Measure IL-8 concentration in cell culture supernatants
  • 13:29Conclusion

Summary

Tadução automática

Tadução automática

高通量小抑制 RNA 筛选是一种重要的工具, 有助于更迅速地阐明化学角膜上皮损伤的分子机制。本文对氟化氢-氯化苦诱导的角膜上皮损伤进行高通量筛选的暴露模型和方法进行了开发和验证。

Tags

High ThroughputSiRNA ScreeningCornea Epithelial CellToxicant InjuryAutomated Liquid HandlingCell SeedingCell Density EvaluationSiRNA Transfection

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